Genome-wide arrayed CRISPR libraries for human gene activation and inhibition
A Webinar by Dr. Jiang-An Yin, University of Zurich, University Hospital Zurich
Unbiased genetic screening is a foundational technique that has been greatly advanced by CRISPR technology. While current CRISPR screens utilize pooled guide RNA libraries, which are limited to selectable phenotypes, arrayed CRISPR libraries significantly expand their scope. However, arrayed CRISPR libraries remain scarce and are challenging to construct.
Utilizing a newly developed massively parallel plasmid cloning methodology, has constructed two genome-wide arrayed libraries for human gene ablation, activation, and epigenetic silencing. Each plasmid encodes an array of four non-overlapping sgRNAs designed to accommodate most human DNA polymorphisms. These new libraries surpass existing resources in both efficiency and versatility across various systems, including induced pluripotent stem cells and organoids. Thus, they unlock vast biological frontiers previously inaccessible and serve as a powerful tool for biomedical research.
Event details:
- Date: 10.04.2025
- Time: 16:00
- Duration: 45 minutes (includes Q&A)
- Location: Online (link to be provided upon registration)
- Costs: Free of charge
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The Dementia Update - Virtual Series is a monthly online event designed to promote knowledge exchange among experts in neurodegenerative disorders. It brings together researchers, clinicians, caregivers and industry professionals to share the latest research, and innovations. Each 45-minute session features a presentation, offering a platform for networking, idea exchange and exploring future collaborations.
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